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KMID : 0365819770170010047
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Journal of Pusan Medical College
1977 Volume.17 No. 1 p.47 ~ p.58
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Histochemical Changes in Lower Leg Muscle Following Compression of Popliteal Artery in Rabbits
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Abstract
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The author observed the histological changes, glycogen distribution and activities of phosphorylase and ATPase in the muscle fibers of gastrocnemius, soleus and tibialis anterior muscles at 1, 2, 4, 8,12, 24 and 48 hours after complete compression with a rubber tourniquet around the popliteal area of the rabbit hind limb. And also examined by using the same methods at 24 and 48 hours of circulatory restoration following release of the tourniquet after 12 hour-compression.
The results obtained were as follows:
1. The histological changes within the circulatorily embarrassed skeletal muscles showed a slight atrophy and degeneration of some fibers within 2 hours following compression(f1g.2) but after 4 hours there could be noted an edema and swelling in muscle bundles, atrophy and hyaline degeneration of the muscle fibers and proliferation of the interfibrillar connective tissue(fig. 3). All the above changes became more pronounced after 8 hours of compression except edema within the muscles which decreased gradually(fig. 4, 5). After 24 hours of compression there were noted rupture and atrophy -of some muscle fibers, a proliferation of the arterioles within the endo- and epimysium with hypertrophy of their walls, and hemorrhage(fig. 6, 7).
2. At 24 hours after release of tourniquet, there could be found hemorrLage, marked change of the blood vessel walls within the muscle bundles and trarked infiltration of polymorplionuclear leukocytes in the endomysium(fig. 8). After 48 hours gradual sub_idecce of the hcrrorrhage and edema in muscles were noted with a gradual restoration of fibrillar appearance (fig. 9).
3. The glycogen content and phosphorylase activity in muscle fibers of the circulatorily embarrassed skeletal muscles disclosed a gradual decrease from 2 hours of compression and complete absence after 4 hours. At 24 to 48 hours after release of tourniquet the PAS reaction and phosphorylase activities reappeared within the muscle fibers.
4. The ATPase activities in large fibers of the circulatorily embarrassed skeletal muscles revealed a slight decrease from 2 to 8 hours of compression but from 12 hours till 48 hours the enzyme activities rather increased within the muscle fibers. In contrast those of small fibers showed neither change nor increases during the compression period. Also no remarkable changes were noted in the recovery stage after release of tourniquet.
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